Browsing by Issue Date, starting with "2023-02"
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- Development of a bench-scale photobioreactor with a novel recirculation system for continuous cultivation of microalgaePublication . Tavares, João; Silva, Tiago; Paixão, Susana M.; Alves, LuísABSTRACT: Microalgae cultivation can be used to increase the sustainability of carbon emitting processes, converting the CO2 from exhaust gases into fuels, food and chemicals. Many of the carbon emitting industries operate in a continuous manner, for periods that can span days or months, resulting in a continuous stream of gas emissions. Biogenic CO2 from industrial microbiological processes is one example, since in many cases it becomes unsustainable to stop these processes on a daily or weekly basis. To correctly sequester these emissions, microalgae systems must be operated under continuous constant conditions, requiring photobioreactors (PBRs) that can act as chemostats for long periods of time. However, in order to optimize culture parameters or study metabolic responses, bench-scale setups are necessary. Currently there is a lack of studies and design alternatives using chemostat, since most works focus on batch assays or semi-continuous cultures. Therefore, this work focused on the development of a continuous bench-scale PBR, which combines a retention vessel, a photocollector and a degasser, with an innovative recirculation system, that allows it to operate as an autotrophic chemostat, to study carbon sequestration from a biogenic CO2-rich constant air stream. To assess its applicability, the PBR was used to cultivate the green microalga Haematococcus pluvialis using as sole carbon source the CO2 produced by a coupled heterotrophic bacterial chemostat. An air stream containing ≈0.35 vol% of CO2, was fed to the system, and it was evaluated in terms of stability, carbon fixation and biomass productivity, for dilution rates ranging from 0.1 to 0.5 d−1. The PBR was able to operate under chemostat conditions for more than 100 days, producing a stable culture that generated proportional responses to the stimuli it was subjected to, attaining a maximum biomass productivity of 183 mg/L/d with a carbon fixation efficiency of ≈39% at 0.3 d−1. These results reinforce the effectiveness of the developed PBR system, making it suitable for laboratory-scale studies of continuous photoautotrophic microalgae cultivation.
- Influence of Increasing Renewable Power Penetration on the Long-Term Iberian Electricity Market PricesPublication . Leal, Pedro; Castro, Rui; Lopes, FernandoABSTRACT: n recent years, there has been a significant increase in investment in renewable energy sources, leading to the decarbonization of the electricity sector. Accordingly, a key concern is the influence of this process on future electricity market prices, which are expected to decrease with the increasing generation of renewable power. This is important for both current and future investors, as it can affect profitability. To address these concerns, a long-term analysis is proposed here to examine the influence of the future electricity mix on Iberian electricity prices in 2030. In this study, we employed artificial intelligence forecasting models that incorporated the main electricity price-driven components of MIBEL, providing accurate predictions for the real operation of the market. These can be extrapolated into the future to predict electricity prices in a scenario with high renewable power penetration. The results, obtained considering a framework featuring an increase in the penetration of renewables into MIBEL of up to 80% in 2030, showed that electricity prices are expected to decrease by around 50% in 2030 when compared to 2019, and there will be a new pattern of electricity prices throughout the year due to the uneven distribution of renewable electricity. The study's findings are relevant for ongoing research on the unique challenges of energy markets with high levels of renewable generation.
- Promoting the Circular Economy on an Island: Anaerobic Co-Digestion of Local Organic Substrates as a Possible Renewable Energy SourcePublication . Fernández-Rodríguez, Juana; Di Berardino, Mónica; Di Berardino, SantinoABSTRACT: The local waste co-digestion is an interesting option to tackle in reduced and isolated areas like the islands. The islands have limited territory and scarce fuel production. Moreover, organic waste can create serious environmental problems in soil, water and air. Anaerobic co-digestion (AcoD) is a technology fulfilling the concept of waste-to-energy (WtE) based on local resources. The valorisation of organic waste through AcoD on an island would prevent environmental impacts, while being a source of renewable energy. In this study, cow manure (outdoor and indoor), pig slurry, bird manure, kitchen waste, sewage sludge and oily lacteous waste produced on Island Terceira (Portugal) were tested in mesophilic -35 degrees C- Biochemical Methane Potential (BMP) co-digestion assays. The goals were to analyse the recalcitrant and high potential produced waste and to estimate the energetic supply source on the island. The cow manure and pig slurry were used as inocula and specific methanogenic activities (SMAs) were carried out. The results showed that both substrates have a significant methanogenic activity-SMA 0.11 g-COD/(g-VSS.d) and 0.085 g-COD/(g-VSS.d), respectively. All the studied combinations were feasible in AcoD, showing TS removals in the range of 19-37%; COD removals in the range 67-78% and specific methane yields from 0.14 to 0.22 L/gCOD removed, but some differences were found. The modified Gompertz model fitted the AcoD assays (R-2 0.982-0.998). The maximum biogas production rate, R-max. was highest in the AcoD of Cow+Pig+Oily and in the Cow+Pig+Sludge with 0.017 and 0.014 L/g-VSadded.day, respectively, and the lowest in Cow+Pig+Bird with 0.010 L/g-VSadded. In our AcoD studies, the bird manure limited the performance of the process, since it was recalcitrant to anaerobic degradation. On the other hand, the oily lacteous waste showed a great potential in the anaerobic digestion. The estimated biogas production, from the best-studied condition, could cover the 11.4% of the energy supply of the inhabitants. These preliminary results would prevent the environmental impact of organic waste on the island and promote the use of local waste in a circular economy scenario.
- Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenasesPublication . Kommedal, Eirik G.; Angeltveit, Camilla F.; Klau, Leesa J.; Ayuso-Fernández, Iván; Arstad, Bjørnar; Antonsen, Simen G.; Stenstrøm, Yngve; Ekeberg, Dag; Gírio, Francisco; Carvalheiro, Florbela; Horn, Svein J.; Aachmann, Finn Lillelund; Eijsink, Vincent G. H.ABSTRACT: Degradation of plant biomass, comprised of cellulose and polyaromatic lignin, is promoted by light. Here, the authors show that light promotes lignin-catalyzed generation of hydrogen peroxide, which is used by redox enzymes to degrade cellulose. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight promotes microbial conversion of plant litter; this effect has been attributed to photochemical degradation of lignin, a major redox-active component of secondary plant cell walls that limits enzyme access to the cell wall carbohydrates. Here, we show that exposing lignin to visible light facilitates cellulose solubilization by promoting formation of H2O2 that fuels LPMO catalysis. Light-driven H2O2 formation is accompanied by oxidation of ring-conjugated olefins in the lignin, while LPMO-catalyzed oxidation of phenolic hydroxyls leads to the required priming reduction of the enzyme. The discovery that light-driven abiotic reactions in Nature can fuel H2O2-dependent redox enzymes involved in deconstructing lignocellulose may offer opportunities for bioprocessing and provides an enzymatic explanation for the known effect of visible light on biomass conversion.
- Environmental Stable and Radioactive Isotopes in the Assessment of Thermomineral Waters in Lisbon Region (Portugal): Contributions for a Conceptual ModelPublication . Marrero Diaz, Rayco; Carvalho, M.R.; Carreira, Paula M.ABSTRACT: The existence of thermomineral waters in the Lisbon region (western Portugal), has been known for centuries. Several authors have investigated the characteristics of these thermomineral waters, but their conceptual model (recharge zone, processes controlling the origin and chemical evolution, depth of circulation, etc.) remains unknown. This work highlights the advantages of assessing the conceptual model in urban coastal areas, where different saline sources coexist, combining chemical analyses with environmental isotopes and geochemical modeling. The final physicochemical composition of thermomineral waters seems to be mainly controlled by water-rock interaction processes and different mixing degrees between HCO3-Ca regional groundwaters, cold dilute shallow groundwaters and highly salinized fluid (brackish or brine or non-recent seawater) probably trapped at depth. Based on these results, neither the contribution of polluted water nor the leaching of evaporite rocks seems to be the main salinization factor of the warmer and saline waters. A positive correlation exists between the lowest radiocarbon activities (average apparent ages similar to 6 ka BP) and highest water temperature and salinity (up to 39 degrees C and 6.3 g/L), suggesting progressive salinization and deeper circulation (up to 1200 m depth) into the hosting formations, where mixing with trapped salinized fluids probably took place, as confirmed by geochemical modeling. Finally, very favorable geological conditions in the Estoril region allow for fast rising and the occurrence of these thermomineral waters at relatively shallow depths.
- Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Methodology for the Novel Microalga Chlorococcum amblystomatisPublication . Correia, Nádia; Pereira, Hugo; Schulze, Peter; Costa, Monya M.; Santo, Gonçalo E.; Guerra, Inês; Trovão, Mafalda; Barros, Ana; Cardoso, Helena; Silva, Joana; Gouveia, Luisa; Varela, JoãoABSTRACT: The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass composition and their biotechnological potential. Media optimization through surface response methodology led to 44.9 and 51.2% increments in C. amblystomatis-specific growth rates under heterotrophic and photoautotrophic growth, respectively. This microalga registered high protein content (61.49-73.45% dry weight), with the highest value being observed in the optimized photoautotrophic growth medium. The lipid fraction mainly constituted polyunsaturated fatty acids, ranging from 44.47 to 51.41% for total fatty acids (TFA) in cells under heterotrophy. However, these contents became significantly higher (70.46-72.82% TFA) in cultures cultivated under photoautotrophy. An interesting carotenoids content was achieved in the cultures grown in optimized photoautotrophic medium: 5.84 mg center dot g(-1) beta-carotene, 5.27 mg center dot g(-1) lutein, 3.66 mg center dot g(-1) neoxanthin, and 0.75 mg center dot g(-1) violaxanthin. Therefore, C. amblystomatis demonstrated an interesting growth performance and nutritional profile for food supplements and feed products that might contribute to meeting the world's nutritional demand.
- Effects of Lignin Gasification Impurities on the Growth and Product Distribution of Butyribacterium methylotrophicum during Syngas FermentationPublication . Pacheco, Marta; Pinto, Filomena; Brunsvik, Anders; Andre, Rui N.; Marques, Paula; Mata, Ricardo; Ortigueira, Joana; Gírio, Francisco; Moura, PatríciaABSTRACT: This work evaluated the effects of condensable syngas impurities on the cell viability and product distribution of Butyribacterium methylotrophicum in syngas fermentation. The condensates were collected during the gasification of two technical lignins derived from wheat straw (WST) and softwood (SW) at different temperatures and in the presence or absence of catalysts. The cleanest syngas with 169 and 3020 ppmv of H2S and NH3, respectively, was obtained at 800 degrees C using dolomite as catalyst. Pyridines were the prevalent compounds in most condensates and the highest variety of aromatics with cyanide substituents were originated during WST lignin gasification at 800 degrees C without catalyst. In contrast with SW lignin-based condensates, the fermentation media supplemented with WST lignin-derived condensates at 1:100 vol. only supported residual growth of B. methylotrophicum. By decreasing the condensate concentration in the medium, growth inhibition ceased and a trend toward butyrate production over acetate was observed. The highest butyrate-to-acetate ratio of 1.3 was obtained by supplementing the fermentation media at 1:1000 vol. with the condensate derived from the WST lignin, which was gasified at 800 degrees C in the presence of olivine. B. methylotrophicum was able to adapt and resist the impurities of the crude syngas and altered its metabolism to produce additional butyrate.
- Como o consumo de eletricidade na indústria está distribuído no território em Portugal continental [Comunicação oral]Publication . Barbosa, Juliana; Simoes, Sofia; Oliveira, Paula; Simões, Teresa; Rodrigues, Carlos; Catarino, Justina; Cardoso, João P.; Pinto, P.J.R.; Quental, Lídia; Patinha, Pedro
- Strategic Bidding of Retailers in Wholesale Markets: Continuous Intraday Markets and Hybrid Forecast MethodsPublication . Algarvio, Hugo; Lopes, FernandoABSTRACT: The deregulation process of the electricity sector has led to competition in wholesale and retail markets. In particular, retailers submit bids to wholesale markets to satisfy the energy needs associated with portfolios of end-use customers. This paper describes a strategic process for retailers bidding in a wholesale market composed of a day-ahead market, an intraday market, and a balancing market. It considers a market design that involves a hybrid model for the intraday market, based on daily auctions and a continuous procedure. The paper also presents a computational study to illustrate and test both the market design and the strategic bidding process of retailers. The results confirm the advantages of considering a continuous intraday market, show that bidding in short-term markets is more beneficial than bidding in medium-term markets, and indicate important aspects to consider when selecting customers to add to the portfolios of retailers.