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- Alternative feedstocks for high-quality biodiesel: Lipid production from eucalyptus bark hydrolysate by Yarrowia lipolytica W29 using different cultivation modesPublication . Dias, Bruna; Lopes, Marlene; Marques, Susana; Gírio, Francisco; Belo, IsabelABSTRACT: Microbial lipids produced by yeasts from lignocellulosic biomass are a promising feedstock for the biodiesel industry, providing a renewable energy source as an alternative to traditional fossil fuels. This study investigated the potential of Yarrowia lipolytica W29 to produce lipid-rich biomass from undetoxified sugar-concentrated eucalyptus bark hydrolysate (EBH). The lipid concentrations achieved in batch cultures (13.4 g L-1) were the highest for wild-type Y. lipolytica strains in lignocellulosic hydrolysates. Different two-stage cultivation modes (repeated batch, continuous-feeding fed-batch, and pulse fed-batch) were studied to enhance biomass and lipid production. The cell and lipid mass was higher in pulse fed-batch and continuous-feeding fed-batch cultures than batch cultures. Production of citric acid, a side product of industrial interest, was improved in the continuous-feeding fed-batch culture. Microbial lipids produced by Y. lipolytica W29 were highly unsaturated and mainly composed of oleic acid (50% to 53%). The estimated properties of the biodiesel that would be obtained from these intracellular lipids would meet the international biodiesel standards EN 14214 and ASTM D6751. This study demonstrates the feasibility of using EBH for Y. lipolytica lipid production and promotes the sustainable production of high-quality biodiesel from lignocellulosic feedstocks.
- Development of a georeferenced database as a tool to improve circular material flows in lignocellulosic waste valorization [Poster]Publication . Silva, Tiago; Eusebio, Ana; Camocho, David; Alexandre, Jorge; Sousa Rocha, Cristina; Patinha, Pedro; Quental, Lídia; Gírio, Francisco; Moura, PatríciaABSTRACT: The Frontsh1p project aims to accelerate the development and implementation of cross-sector circular value chains through the creation of a multi-level toolkit that includes georeferenced data. This will facilitate the identification of the different stakeholders, their levels of interaction, regional distribution, and communication pathways, as well as the planning of new circular material flows for by-products and waste streams. The region of Łódzkie in Poland was selected for the development of this toolkit to establish guidelines, methodologies and technologies, based on information at the regional economic level, that can be easily replicated in different regions across Europe.
- Integrating social aspects in microalgal biorefineries: a Product Social Impact Life Cycle Assessment (PSILCA) approachPublication . Ortigueira, Joana; Lopes, Tiago; Reis, Alberto; Gírio, FranciscoABSTRACT: The ongoing climate change phenomenon requires the reduction of atmospheric CO2 concentrations. Microalgal biorefineries, which convert atmospheric CO2 into chemical energy, offer a viable alternative to fossil fuel-based industrial systems. This study assesses the social impacts of microalgal biorefineries using the Product Social Impact Life Cycle Assessment database approach, focusing on an industrial facility located in P & oacute;voa de Santa Iria, Vila Franca de Xira, Portugal. The foreground system involves the production of microalgae in cascade raceway systems, followed by their refinement into protein, lipid and carbohydrate fractions. Dedicated surveys were distributed to the local community to collect social data, which was then analyzed using the Product Social Impact Life Cycle Assessment (PSILCA) database and a newly designed evaluation schema. Preliminary data from approximately 300 valid responses indicated that the local community faces a medium risk of being unfamiliar with the concept of microalgae or its benefits but acknowledged the high probability of local economic benefits and job creation upon implementation. The study highlights a general lack of familiarity with microalgae among the local community, which could affect the acceptance of the biorefinery. Although the PSILCA approach identifies social hotspots effectively, reliance on generic data may not accurately represent the local context. The study underscores the need for enhanced information dissemination to improve community acceptance and support for microalgal biorefineries. Preliminary data collection and analysis highlight the potential for social benefits, but further research is required to address the identified limitations.