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Research Project
Biorremediação de efluentes agroindustriais por microalgas e valorização da biomassa algal numa economia circular
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A circular approach for landfill leachate treatment: chemical precipitation with biomass ash followed by bioremediation through microalgae
Publication . Viegas, Catarina; Nobre, Catarina; Mota, André; Vilarinho, Cândida; Gouveia, Luisa; Gonçalves, Maria Margarida
ABSTRACT: The aim of this work was to study an integrated approach for landfill leachate remediation comprising chemical precipitation with biomass bottom ash as a pre-treatment to reduce color and turbidity followed by bioremediation through microalgae treatment for effluent disposal. Optimal pre-treatment conditions were determined through batch experiments and were found to be 160 g L-1 ash dose, 96 h of contact time, overhead agitation at 15 rpm and ash particle size below 500 mu m. These conditions led to removal efficiencies of 74.3% for chemical oxygen demand and 98.5% for color. Large quantities of sludge containing excess biomass ash and precipitated compounds were formed during the pre-treatment. To minimize solid disposal, this sludge was tested as a raw material for cementitious and aggregate substitute in mortar formulations. Following the pre-treatment, the leachate was inoculated with six different microalgae species to evaluate their ability to grow in such a recalcitrant effluent and remediate it. After a period of 27 days biomass concentration from 0.4 to 1.2 g L-1 were achieved for the tested microalgae. Removal efficiencies were in the range of 18-62% for COD, 63-71% for N, and 15-100% for P. At the end of the treatment, algal biomass was characterized regarding protein, lipid, fatty acids, carbohydrate, and ash contents. This approach allows a low-cost remediation of these recalcitrant effluents when compared with the present options that include inverse osmosis, and the valorization of ash-rich precipitates and microalgae biomass improves the sustainability of the overall process.
Aquaculture wastewater treatment through microalgal: biomass potential applications on animal feed, agriculture, and energy
Publication . Viegas, Catarina; Gouveia, Luisa; Gonçalves, Maria Margarida
ABSTRACT: The use of microalgae to remediate raw effluent from brown crab aquaculture was evaluated by performing batch mode growth tests using separately the microalgae Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc), Isochrysis galbana (Ig), Nannocloropsis salina (Ns), and Spirulina major (Sp). Removal efficiencies in batch growth were 100% for total nitrogen and total phosphorus for all microalgae. Chemical oxygen demand (COD) remediations were all above 72%. Biomass productivity varied from 20.9 mg L-1 day- 1 (N. salina) to 146.4 mg L-1 day- 1 (C. vulgaris). The two best performing algae were C. vulgaris and S. obliquus and they were tested in semicontinuous growth, reaching productivities of 879.8 mg L-1 day- 1 and 811.7 mg L-1 day- 1, respectively. The bioremediation of the effluent was tested with a transfer system consisting of three independent containers and compared with the use of a single container. The single container had the same capacity and received weekly the same volume of effluent as the three containers together. The remediation capacity of the 3 containers was much higher than the single one. The supplementation with NaNO3 was tested to improve the nutrient removal microalgae? capacity, with positive results. The removal efficiencies were 100% for total nitrogen and total phosphorus and higher than 96% for COD. The obtained C. vulgaris and S. obliquus biomass were composed of 31 and 35% proteins, 6 and 8% lipids, 39 and 30% carbohydrates, respectively. The composition of these biomass suggest that it can be used as novel and sustainable ingredients in aquaculture feeds. The algal biomass of Cv and Sc were used as biostimulants in the germination of wheat and watercress, and very promising results were attained, with increases in the germination index for Cv and Sc of 175% and 48% in watercress and 84% and 98% in wheat, respectively. The biomasses of Cv and Sc were also subjected to a torrefaction process with 72.5 ? 1.7% char yields. The obtained biochars were tested as biostimulants for germination seeds (wheat and watercress) and as bio-adsorbent of dye solutions.
Optimization of Biochar Production by Co-Torrefaction of Microalgae and Lignocellulosic Biomass Using Response Surface Methodology
Publication . Viegas, Catarina; Nobre, Catarina; Correia, Ricardo; Gouveia, Luisa; Gonçalves, Maria Margarida
ABSTRACT: Co-torrefaction of microalgae and lignocellulosic biomass was evaluated as a method to process microalgae sludge produced from various effluents and to obtain biochars with suitable properties for energy or material valorization. The influence of four independent variables on biochar yield and properties was evaluated by a set of experiments defined by response surface methodology (RSM). The biochars were characterized for proximate and ultimate composition, HHV, and methylene blue adsorption capacity. HHV of the biochars was positively correlated with carbonization temperature, residence time, and lignocellulosic biomass content in the feed. Co-torrefaction conditions that led to a higher yield of biochar (76.5%) with good calorific value (17.4 MJ Kg(-1)) were 250 & DEG;C, 60 min of residence time, 5% feed moisture, and 50% lignocellulosic biomass. The energy efficiency of the process was higher for lower temperatures (92.6%) but decreased abruptly with the increase of the moisture content of the feed mixture (16.9 to 57.3% for 70% moisture). Biochars produced using algal biomass grown in contaminated effluents presented high ash content and low calorific value. Dye removal efficiency by the produced biochars was tested, reaching 95% methylene blue adsorption capacity for the biochars produced with the least severe torrefaction conditions.Co-torrefaction of microalgae and lignocellulosic biomass was evaluated as a method to process microalgae sludge produced from various effluents and to obtain biochars with suitable properties for energy or material valorization. The influence of four independent variables on biochar yield and properties was evaluated by a set of experiments defined by response surface methodology (RSM). The biochars were characterized for proximate and ultimate composition, HHV, and methylene blue adsorption capacity. HHV of the biochars was positively correlated with carbonization temperature, residence time, and lignocellulosic biomass content in the feed. Co-torrefaction conditions that led to a higher yield of biochar (76.5%) with good calorific value (17.4 MJ Kg(-1)) were 250 & DEG;C, 60 min of residence time, 5% feed moisture, and 50% lignocellulosic biomass. The energy efficiency of the process was higher for lower temperatures (92.6%) but decreased abruptly with the increase of the moisture content of the feed mixture (16.9 to 57.3% for 70% moisture). Biochars produced using algal biomass grown in contaminated effluents presented high ash content and low calorific value. Dye removal efficiency by the produced biochars was tested, reaching 95% methylene blue adsorption capacity for the biochars produced with the least severe torrefaction conditions.
Bioremediation of cattle manure using microalgae after pre-treatment with biomass ash
Publication . Viegas, Catarina; Gouveia, Luisa; Margarida Gonçalves, Maria
ABSTRACT: In this work, cattle manure was diluted and pre-treated with biomass ash to yield a liquid fraction and a solid precipitate. Microalgae grown in the liquid fraction, in batch and semi-continuous mode, achieved maximum biomass productivities of 522.9 and 554.3 mg L−1 day−1 (12 days) for Chlorella protothecoides and Tetradesmus obliquus, respectively. Nutrient removal efficiency was highest for the semicontinuous mode with replacement of 10% of reactor volume every 48 h. The produced algal biomass was characterized for its nutrient composition. Both, algal biomass, and precipitate aqueous extracts, were evaluated as biostimulants for wheat and watercress seeds Increments in the germination index were 177% for wheat with Chlorella protothecoides and 34% for watercress with Tetradesmus obliquus. The strategy adopted in this work is coherent with circular economy principles, combining effluent treatment with the production of added-value materials that could be used as biostimulants or animal feed additives.
Evaluation of microalgae as bioremediation agent for poultry effluent and biostimulant for germination
Publication . Viegas, Catarina; Gouveia, Luisa; Gonçalves, Maria Margarida
ABSTRACT: This work addresses how a pre-treatment involving biomass ash influences the poultry effluent's bioremediation using three microalga strains, such as Chlorella vulgaris, Chlorella protothecoides and Tetradesmus obliquus. The undiluted effluent served as the culture medium for the growth, both in batch and semi continuous modes, and the remediation efficiency and biomass production yield were quantified. The combination strategy in batch mode, allowed removal efficiency of 100% for total nitrogen, more than 80% for total phosphorus and over 70% for chemical oxygen demand. Average biomass productivities for 10 days of 94.9, 76.2 and 72.0 mg L-1 day(-1) were obtained for T. obliquus, C. vulgaris and C. protothecoides, respectively. Regarding semi-continuous strategy (28 days), the biomass productivities achieved were 245 and 194 mg L-1 day(-1) for T. obliquus and C. vulgaris, respectively. Remediation rates of 100% for total nitrogen and phosphorus, and over 92% for COD were attained. The microalga composition was assessed for protein, sugar, lipid, and ash contents. The produced biomasses were tested as biostimulant and showed a 147% increase in wheat germination index, for the C. vulgaris microalga. The use of the precipitate from the biomass ash pre-treatment as fertilizer in germination tests was also assessed and results in an increase of 26%, for 10% of precipitate incorporation.
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Fundação para a Ciência e a Tecnologia
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SFRH/BD/131178/2017