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Recovery of bioactive compounds from industrial exhausted olive pomace through ultrasound-assisted extraction

2021-06Journal article Open access
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dc.contributor.authorGómez Cruz, Irene
dc.contributor.authorContreras, Maria del Mar
dc.contributor.authorCarvalheiro, Florbela
dc.contributor.authorDuarte, Luís C.
dc.contributor.authorRoseiro, Luisa B.
dc.contributor.authorRomero, Inmaculada
dc.contributor.authorCastro, Eulogio
dc.date.accessioned2021-12-13T12:46:17Z
dc.date.available2021-12-13T12:46:17Z
dc.date.issued2021-06
dc.description.abstractABSTRACT: Simple Summary Exhausted olive pomace (EOP) is the main residue of the pomace oil extraction industry, which is generated in large quantities and has limited applications. Thus, this study aimed to obtain bioactive compounds from EOP using ultrasound-assisted extraction as a potential first valorization step. Two types of devices were tested: bath- and probe-type UAE. The operational parameters were studied and optimized to maximize the antioxidant compounds. In particular, hydroxytyrosol was the main phenolic compound identified and its content was 5.16 mg/g EOP (bath-type UAE) and 4.96 mg/g EOP (probe-type UAE). Mannitol was also detected in the extract, 59.53 mg/g EOP (bath-type UAE) and 69.73 mg/g EOP (probe-type UAE). The results highlight the great potential EOP has as a source of bioactive compounds, with applicability in several sectors. Moreover, the probe-type UAE shows potential to be applied for obtaining these bioactive compounds in a continuous and faster manner. Exhausted olive pomace (EOP) is the main agro-industrial waste of the olive pomace extracting industries. It contains phenolic compounds and mannitol, so the extraction of these bioactive compounds should be considered as a first valorization step, especially if EOP is used as biofuel. Therefore, EOP was subjected to bath-type ultrasound-assisted extraction (UAE), and the effects of the acetone concentration (20-80%, v/v), solid load (2-15%, w/v), and extraction time (10-60 min) on the extraction of antioxidant compounds were evaluated according to a Box-Behnken experimental design. By means of the response surface methodology, the optimum conditions were obtained: 40% acetone, 8.6% solids, and 43 min. For all the extracts, the total phenolic content (TPC), flavonoid content (TFC), and antioxidant activity (DPPH, ABTS, and FRAP) were determined. With the aim of shortening the extraction time, a two-level factorial experiment design was also carried out using a probe-type UAE, keeping the solid load at 8.6% (w/v) and the acetone concentration at 40% (v/v), while the amplitude (30-70%) and the extraction time (2-12 min) were varied to maximize the aforementioned parameters. Finally, a maximum of phenolic compounds was reached (45.41 mg GAE/g EOP) at 12 min and 70% amplitude. It was comparable to that value obtained in the ultrasonic bath (42.05 mg GAE/g EOP), but, remarkably, the extraction time was shortened, which translates into lower costs at industrial scale. Moreover, the bioactive compound hydroxytyrosol was found to be the major phenolic compound in the extract, i.e., 5.16 mg/g EOP (bath-type UAE) and 4.96 mg/g EOP (probe-type UAE). Other minor phenolic compounds could be detected by capillary zone electrophoresis and liquid-chromatography-mass spectrometry. The sugar alcohol mannitol, another bioactive compound, was also found in the extract, and its content was determined. Thus, the use of this technology can support the valorization of this waste to obtain bioactive compounds, including mannitol, hydroxytyrosol, and other derivatives, before being applied for other uses.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGómez-Cruz, Irene... [et.al.] - Recovery of bioactive compounds from industrial exhausted olive pomace through ultrasound-assisted extraction. In: Biology, 2021, Vol.10(6), article nº 514pt_PT
dc.identifier.doi10.3390/biology10060514pt_PT
dc.identifier.eissn2079-7737
dc.identifier.urihttp://hdl.handle.net/10400.9/3628
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation(BBRI)-LISBOA-01-0145-FEDER-022059 (PORTUGAL 2020)pt_PT
dc.relation.publisherversionhttps://doi.org/10.3390/biology10060514pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOlive pomacept_PT
dc.subjectAgricultural Residuespt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectWaste valorizationpt_PT
dc.titleRecovery of bioactive compounds from industrial exhausted olive pomace through ultrasound-assisted extractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume10pt_PT
person.familyNameGómez Cruz
person.familyNameCONTRERAS
person.familyNameCarvalheiro
person.familyNameDuarte
person.familyNamede Bivar Weinholtz Roseiro
person.familyNameROMERO
person.familyNameCastro Galiano
person.givenNameIrene
person.givenNameMARIA DEL MAR
person.givenNameFlorbela
person.givenNameLuís
person.givenNameMaria Luisa
person.givenNameINMACULADA
person.givenNameEulogio
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person.identifier.orcid0000-0002-0270-4832
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person.identifier.orcid0000-0003-1562-6658
person.identifier.orcid0000-0002-4152-8034
person.identifier.orcid0000-0003-1719-6049
person.identifier.ridL-1860-2014
person.identifier.ridC-1430-2008
person.identifier.ridL-1101-2014
person.identifier.ridC-2181-2012
person.identifier.ridE-5910-2011
person.identifier.scopus-author-id26653952000
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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