Publication
Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii: cell-cell contact mechanism
| dc.contributor.author | Branco, Patricia | |
| dc.contributor.author | Kemsawasd, Varongsiri | |
| dc.contributor.author | Arneborg, Nils | |
| dc.contributor.author | Albergaria, Helena | |
| dc.date.accessioned | 2014-04-08T16:26:27Z | |
| dc.date.available | 2014-04-08T16:26:27Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Several studies have shown that the early death of non-Saccharomyces during wine fermentations are due to yeast-yeast interactions induced by Saccharomyces cerevisiae (Sc) through different mechanisms such as growth arrest mediated by a cell-cell contact mechanism (Nissen et al. 2003) and death mediated by killer-like toxins (Pérez-Nevado et al 2006; Albergaria et al. 2010). Besides, previous work also showed that death of non-Saccharomyces in co-cultivation with Sc is always triggered at the end of exponential growth phase (Pérez-Nevado et al 2006). In order to investigate the role of cell-cell contact in the early death of non-Saccharomyces, we performed assays in which Sc cells pre-grown at enological growth conditions for 12 and 48 h, respectively, were in direct contact with Hanseniaspora guilliermondii (Hg) cells at high cellular density (107-108 cells/ml) in a carbon-free medium. As a negative control we performed similar assays in which Sc and Hg cells were separated by a dialysis tube (pore cut-off of 1000 kDa) and as a positive control a single Hg culture. Results showed that Hg cell density decreased by 2 orders of magnitude (i.e. from 108-106 cells/ml) in contact with 48 h-grown Sc cells, while its viability remained unchanged (108cfu/ml) in the presence of 12 h-grown Sc cells. Moreover, Hg viability was not affected both in the dialysis tube experiments and single culture, which confirmed the death-induced cell-cell contact phenomenon. | por |
| dc.identifier.citation | Branco, P.; Kemsawasd, V.; Arneborg, N.; Albergaria, H. Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii: cell-cell contact mechanism. In: MicroBiotec'13: Portuguese Congress of Microbiology and Biotechnology, Book of Abstracts, Aveiro, Portugal, 6-8 December, 2013, p. 324 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.9/2398 | |
| dc.language.iso | eng | por |
| dc.subject | Saccharomyces cerevisiae | por |
| dc.subject | Hanseniaspora guilliermondii | por |
| dc.subject | Wine fermentations | por |
| dc.title | Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii: cell-cell contact mechanism | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Aveiro, Portugal | por |
| oaire.citation.title | MicroBiotec'13: Portuguese Congress of Microbiology and Biotechnology | por |
| person.familyName | Batista Branco | |
| person.familyName | Albergaria | |
| person.givenName | Patricia Alexandra | |
| person.givenName | Helena | |
| person.identifier.ciencia-id | F314-7279-6F0F | |
| person.identifier.ciencia-id | FF10-6CD1-AF19 | |
| person.identifier.orcid | 0000-0002-1760-6899 | |
| person.identifier.orcid | 0000-0002-9062-9827 | |
| person.identifier.rid | A-8351-2015 | |
| person.identifier.scopus-author-id | 7801441357 | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |
| relation.isAuthorOfPublication | 5f614149-d826-4268-bdbf-bc3a938d5d42 | |
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| relation.isAuthorOfPublication.latestForDiscovery | 63df14e3-952f-4b43-b37c-3cc1238e0830 |