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  • Biocatalytic performance of Butyribacterium methylotrophicum in the long-term conversion of synthesis gas produced from low-grade lignin gasification by Butyribacterium methylotrophicum [Resumo]
    Publication . Pacheco, Marta; Pinto, Filomena; Andre, Rui N.; Marques, Paula; Gírio, Francisco; Moura, Patrícia
    ABSTRACT: Second-generation biorefineries produce large streams of low-grade lignin. Its thermochemical conversion, through gasification, enables the carbon recovery from an otherwise recalcitrant by-product. The main product of gasification is producer synthesis gas (PS), which is mainly composed by carbon monoxide (CO), carbon dioxide (CO2), hydrogen (H2), methane (CH4) and minor impurities. Carboxydotrophic acetogenic bacteria can utilize CO and CO2 as carbon and energy source, and convert them into biomass, biofuels and biochemicals through the Wood-Ljungdahl pathway.
  • Anaerobic digestion of enzymatically treated dairy cow effluent [Resumo]
    Publication . Eusebio, Ana; Marques, Susana; Marques, Isabel Paula
    ABSTRACT: In Portugal, cattle production increased up to 91,000 t in 2016 (EUROSTAT, 2016) and has been contributing to the increase of methane emissions into the atmosphere. As an appropriate process for the treatment of organic effluents, anaerobic digestion is ideal to minimize this negative impact since it provides the agricultural and energetic valorisation of the substrate through the production of digestate and biogas. Heating, cooling and electricity supply constitute different applications for this energy carrier gas with a relevant social, environmental and economic importance, mainly for the region and neighbouring areas where it is produced. Dairy cow effluent is an example of lignocellulosic biomass, whose recalcitrant compounds, such as lignin and phenols, must be previously hydrolysed and made available for subsequent anaerobic digestion. The use of laccase and hemicellulase may be of great interest as pre-treatment for a wide range of pollutant compounds of effluents.
  • Anaerobic digestion of pre-treated microalgae biomass [Resumo]
    Publication . Neves, André; Silva, Teresa Lopes da; Reis, Alberto; Ramalho, Luís; Eusebio, Ana; Marques, Isabel Paula
  • Energetic potential of piggery effluent by anaerobic digestion [Resumo]
    Publication . Eusebio, Ana; Ramalho, Luís; Carvalheiro, Florbela; Gírio, Francisco; Marques, Isabel Paula
    ABSTRACT: The sector of pig farming in Portugal is looking for sustainable and practical solutions to overcome existing environmental problems caused by very polluting discharges of effluents in rivers and wastewater treatment plants. The anaerobic digestion (AD) is a suitable process used to treat an organic effluent and, simultaneously, provide the agricultural and energetic valorisation of the substrate. The main objective of this work was to evaluate the energetic potential of a piggery effluent, collected in a pig farm with 9,000 animals, estimated in a volume of 900-1,170 m3 d-1. Anaerobic digestion assays were carried out in batch mode, under anaerobic and mesophilic conditions.
  • Potencialidades da espetrometria de fluorescência de raios x na área da energia
    Publication . Crujeira, Teresa; Trancoso, Maria Ascensão
    RESUMO: A espectrometria de fluorescência de raios X em dispersão de comprimentos de onda (FRX-DCO) é uma técnica analítica comparativa, não destrutiva, que permite uma abordagem expedita para elementos de número atómico entre 9 e 92, que apresenta um baixo risco de contaminação e que é aplicável a diversos tipos de matrizes.
  • Rede de laboratórios para a energia e geologia
    Publication . Roseiro, J. Carlos; Trancoso, Maria Ascensão; Cunha Diamantino, Teresa; Carvalho, Maria João; Machado Leite, Mário
    RESUMO: O Laboratório Nacional de Energia e Geologia (LNEG), nas suas áreas de competências reuniu as competências de anteriores instituições com um histórico de trabalho em Laboratórios com acreditação desde os anos 80. No LNEG, estes Laboratórios focaram-se em atividades da área de energia e geologia, mantendo as suas acreditações mas sentiram a necessidade de trabalhar em rede.
  • Avaliação da conformidade do teor em enxofre em gasóleo e biodiesel
    Publication . Crujeira, Teresa; Calisto, Sandra C.; Trancoso, Maria Ascensão
    RESUMO: Os transportes rodoviários que utilizam combustíveis de origem fóssil são uma das principais fontes de emissão de poluentes para a atmosfera, conduzindo à degradação da qualidade do ar, em particular nos grandes centros urbanos. A necessidade de utilização de combustíveis de origem renovável levou ao desenvolvimento de combustíveis alternativos, que permitem a redução significativa de emissões de CO2, ou a adição de produtos de origem renovável ao gasóleo. No caso dos óxidos de enxofre, a redução dessas emissões tem sido conseguida como resultado da progressiva diminuição do teor máximo de enxofre permitido nos combustíveis rodoviários.
  • Low-energy harvesting and drying methods for Spirulina maxima: effect on phycocyanin content [Resumo]
    Publication . Batista, Ana Paula; Monteiro, Ana Carina; Loureiro, David; Gouveia, Luisa; Sousa, Isabel
  • Carbon dioxide biofixation and lipid accumulation by green microalgae species at different CO2 concentrations [Resumo]
    Publication . Leonardo, Joana; Batista, Ana Paula; Manoel, João; Reis, Alberto; Marques, Paula; Gouveia, Luisa
  • Food and fuel microalgae applications: insights from portuguese experience [Resumo]
    Publication . Batista, Ana Paula; Nobre, B. P.; Oliveira, Ana Cristina; Passarinho, Paula; Marques, Paula; Ribeiro, Belina; Raymundo, Anabela; Sousa, Isabel; Gouveia, Luisa; Marques, Isabel Paula
    Microalgae have a wide range of application fields, from food to fuels, to pharmaceuticals & fine chemicals, aquaculture and environmental bioremediation, among others. Spirulina and Chlorella have been used as food sources since ancient times, due to their high and balanced nutritional value. Our research group in Lisbon has developed a range of food products (emulsions, gelled desserts, biscuits and pastas) enriched with freshwater and marine microalgae (Spirulina, Chlorella, Haematococcus, Isochrysis and Diacronema). The developed products presented attractive and stable colours, high resistance to oxidation and enhanced rheological properties. Some of these products will be prepared at the Post-Congress Course “Functional Foods Development” at the University of Antofagasta. More recently, a great interest has arisen on using microalgae for biofuel production. The same group has also been exploring several marine and freshwater species for biofuel production (e.g., biodiesel, bioethanol, biohydrogen and biomethane) within a biorefinery approach, in order to obtain high and low-value co-products using integral biomass maximizing the energy revenue. Namely, supercritical fluid extraction of Nannochloropsis sp. allowed the recovery of valuable carotenoids and lipids, prior to bioH2 production through dark fermentation of the residual biomass. Also, Scenedesmus obliquus residues after sugars (for bioethanol) and lipids (for biodiesel) extraction has been anaerobically digested attaining high biomethane yields. Regarding sustainability issues, the current trend of our group is now focused on using liquid effluents and high CO2 levels for low cost microalgae growth, contributing to a lower water demand, primary energy consumption and global warming potential by reducing the need for potable water and fertilizers (P, N) and increasing CO2 mitigation. Microalgae biomass has been successfully used for urban wastewater treatment with subsequent bioH2 production, in a biorefinery approach. Presently, ammonium-rich raw effluents from piggeries and poultry industry are being effectively used for microalgae growth avoiding any pre-treatment step.