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Nanobubble-enhanced oxygen transfer in bacterial nanocellulose production: Comparative evaluation with static and airlift systems

2026-02Artigo científico Acesso aberto
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorRodrigues, Ana Cristina
dc.contributor.authorMartins, Daniela
dc.contributor.authorCarvalho, Ricardo
dc.contributor.authorMarques, Susana
dc.contributor.authorBelo, Isabel
dc.contributor.authorEspina, Begona
dc.contributor.authorDourado, Fernando
dc.contributor.authorGama, Miguel
dc.date.accessioned2026-03-10T17:16:22Z
dc.date.available2026-03-10T17:16:22Z
dc.date.issued2026-02
dc.description.abstractABSTRACT: Despite the unique properties of bacterial nanocellulose (BNC), oxygen limitation during large-scale production impairs microbial metabolism and cellulose synthesis, leading to high production costs and limited commercial success. Static fermentation can achieve high titers, but industrially it is operationally challenging. Agitated systems like airlift (AL) bioreactors, allow faster production but typically yield lower titers. This study pioneered the use of an agitated bioreactor equipped with a nanobubble (NB) generator, and its performance was compared with that of static and AL systems, employing a newly isolated Komagataeibacter sp. strain from kombucha cultivated in Eucalyptus bark hydrolysate and corn steep liquor. Key monitored parameters included dissolved oxygen, cell density, pH, sugar and lactic acid contents, and BNC production. The obtained BNC was characterized for its crystallinity, thermal stability, degree of polymerization, morphology and fiber size. The AL-and NB-derived BNC exhibited a denser network structure, lower crystallinity index, and lower polymerization degrees than that from static culture. NB technology generated stable nanobubbles (size: 95.8 f 12.9 nm; zeta potential:-14.2 f 8.6 mV). At 1 L. min-1 airflow, compared to AL, the NB bioreactor achieved a 6-fold higher volumetric mass transfer coefficient (kLa 35.9 f 1.2 h-1) and oxygen transfer rate (OTR: 309.7 f 10.2 mg.L-1.h-1). It supported greater cell density but maintained a similar BNC volumetric productivity to that of the AL (0.023 g.L-1.h-1), and moderately higher (near 280%) than that of static culture (0.0082 g.L-1.h-1). Thus, the improved oxygen levels provided by the NB system favored biomass growth rather than BNC production, suggesting that further optimization is needed to redirect carbon flux toward BNC production.eng
dc.identifier.citationRodrigues, A.C., Martins, D., Carvalho, R., Marques, S., Belo, I., Espiña, B., Dourado, F., & Gama, M. (2026). Nanobubble-enhanced oxygen transfer in bacterial nanocellulose production: Comparative evaluation with static and airlift systems. In: International Journal of Biological Macromolecules, 2026, vol. 346, article 150572. https://doi.org/10.1016/j.ijbiomac.2026.150572
dc.identifier.doi10.1016/j.ijbiomac.2026.150572
dc.identifier.eissn1879-0003
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.9/6299
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
dc.relationEnhancing Bacterial Cellulose Production through Metabolic Engineering and Nanobubble Technology for Cosmetic Applications
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0141813026004988?pes=vor&utm_source=clarivate&getft_integrator=clarivate
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEucalyptus bark hydrolysate
dc.subjectBacterial nanocellulose
dc.subjectNanobubbles bioreactor
dc.subjectAirlift bioreactor
dc.titleNanobubble-enhanced oxygen transfer in bacterial nanocellulose production: Comparative evaluation with static and airlift systemseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04469/2020
oaire.awardNumberLA/P/0029/2020
oaire.awardNumber2023.17173.ICDT
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
oaire.awardTitleEnhancing Bacterial Cellulose Production through Metabolic Engineering and Nanobubble Technology for Cosmetic Applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIhttp://hdl.handle.net/10400.9/5627
oaire.awardURIhttp://hdl.handle.net/10400.9/6298
oaire.citation.titleInternational Journal of Biological Macromolecules
oaire.citation.volume346
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de Projetos de IC&DT em Todos os Domínios Científicos 2023
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues
person.familyNameMartins
person.familyNameCarvalho
person.familyNameMarques
person.familyNameBelo
person.givenNameAna Cristina
person.givenNameDaniela
person.givenNameRicardo
person.givenNameSusana
person.givenNameIsabel
person.identifier.orcid0000-0002-5015-6842
person.identifier.orcid0000-0001-8125-8326
person.identifier.orcid0000-0003-3120-3727
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-6747-8389
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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