Publication
Zygosaccharomyces bailii strain talf1 inulinases/invertases towards enhanced bioprocesses
| dc.contributor.author | Paixão, Susana M. | |
| dc.contributor.author | Arez, B. F. | |
| dc.contributor.author | Silva, Tiago | |
| dc.contributor.author | Teixeira, Pedro D. | |
| dc.contributor.author | Alves, Luís | |
| dc.date.accessioned | 2015-01-08T15:34:18Z | |
| dc.date.available | 2015-01-08T15:34:18Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Zygosaccharomyces bailii strain Talf1, a yeast recently isolated in our laboratory, produces a crude enzymatic extract that was determined to have both inulinase/ invertase activities. The properties of these activities were characterized and the crude extract was applied towards bioprocesses. The extracellular crude enzymatic extract presents activity in a range of temperatures from 20 to 60°C and pH from 4.5 to 7. Optimal pH and temperature was 5.5 and 50°C for both activities, with long-term thermostability at 25-30°C and pH 5.5. Maximal activities on crude were obtained when Jerusalem artichoke (JA) was used as inducer, attaining 18 and 156 U/ml of inulinase and invertase activities, respectively. Z. bailii Talf1 was used for bioethanol production through consolidated bioprocessing using JA juice as the only nutrients medium (~130 g/l total hydrolysable sugars), producing 67 g/l of ethanol and attaining the maximum theoretical yield (0.51 g/g hydrolysable sugars). Moreover, the crude enzymatic extract was applied for fossil fuels biodesulfurization. Thus, two renewable agro-materials, sugar beet molasses (SBM) and JA, were exploited as cheaper carbon sources for a cost-effective dibenzothiophene desulfurization process by Gordonia alkanivorans strain 1B through simultaneous saccharification and fermentation (SSF) approach. SSF with invertases/inulinases permitted an increase in 2-hydroxybiphenyl specific production rate of 19.5% from SBM (2.61 to 3.12 µmol g-1DCW h-1) and 64.6% (5.06 to 8.33 µmol g-1DCW h-1) from JA juice in comparison to acidic hydrolysis. These results highlight the potential of Talf1 as a CBP strain towards bioethanol and of its enzymatic crude towards enhanced biodesulfurization. | por |
| dc.identifier.citation | Paixão, S.M.; Arez, B.F.; Silva, T.P.; Teixeira, T.D.; Alves, L. - Zygosaccharomyces bailii strain talf1 inulinases/invertases towards enhanced bioprocesses. In: RRB'10-10th International Conference on Renewable Resources and Biorefineries, Book of Abstracts, Valladolid, Spain, 4-6 June, 2014, p. 61 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.9/2570 | |
| dc.language.iso | eng | por |
| dc.subject | Zygosaccharomyces bailii | por |
| dc.subject | Bioprocess | por |
| dc.subject | Biodesulfurization | por |
| dc.title | Zygosaccharomyces bailii strain talf1 inulinases/invertases towards enhanced bioprocesses | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Valladolid, Spain | por |
| oaire.citation.title | RRB'10-10th International Conference on Renewable Resources and Biorefineries | por |
| person.familyName | Paixão | |
| person.familyName | Silva | |
| person.familyName | Alves | |
| person.givenName | Susana M. | |
| person.givenName | Tiago | |
| person.givenName | Luís | |
| person.identifier.ciencia-id | 7918-C133-C5FB | |
| person.identifier.ciencia-id | E418-9EE4-9BB4 | |
| person.identifier.ciencia-id | 561B-53A5-7359 | |
| person.identifier.orcid | 0000-0003-0955-4467 | |
| person.identifier.orcid | 0000-0003-0120-557X | |
| person.identifier.orcid | 0000-0001-6245-775X | |
| person.identifier.scopus-author-id | 6603112228 | |
| person.identifier.scopus-author-id | 6701310833 | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |
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| relation.isAuthorOfPublication.latestForDiscovery | b763d40e-827e-4b6b-949c-d6c8a7166cc5 |