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Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry

2021-02Journal article Open access
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dc.contributor.authorTaborda, Tiago
dc.contributor.authorMoniz, Patricia
dc.contributor.authorReis, Alberto
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2021-12-03T12:15:47Z
dc.date.available2021-12-03T12:15:47Z
dc.date.issued2021-02
dc.description.abstractABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTaborda, Tiago... [et.al.] - Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry. In: Journal of Applied Phycology, 2021, Vol. 33 (1), p. 263-274pt_PT
dc.identifier.doi10.1007/s10811-020-02304-zpt_PT
dc.identifier.eissn1573-5176
dc.identifier.issn0921-8971
dc.identifier.urihttp://hdl.handle.net/10400.9/3624
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://doi.org/10.1007/s10811-020-02304-zpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicroalgaept_PT
dc.subjectAlternative carbon sourcept_PT
dc.subjectSubstratespt_PT
dc.subjectLipidspt_PT
dc.subjectFlow cytometrypt_PT
dc.titleEvaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30169%2F2017/PT
oaire.citation.endPage274pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage263pt_PT
oaire.citation.titleJournal of Applied Phycologypt_PT
oaire.citation.volume33pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameGamito Taborda
person.familyNameMoniz
person.familyNameDelgado dos Reis
person.familyNameSaraiva Lopes da Silva
person.givenNameTiago Miguel
person.givenNamePatricia
person.givenNameAlberto José
person.givenNameMaria Teresa
person.identifier.ciencia-id3518-9ADB-7E93
person.identifier.ciencia-idEC1A-602C-C41D
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0002-7757-8746
person.identifier.orcid0000-0002-7085-674X
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-4365-8868
person.identifier.scopus-author-id37085399000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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