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Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
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Advisor(s)
Abstract(s)
ABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
Description
Keywords
Microalgae Alternative carbon source Substrates Lipids Flow cytometry
Pedagogical Context
Citation
Taborda, Tiago... [et.al.] - Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry. In: Journal of Applied Phycology, 2021, Vol. 33 (1), p. 263-274
Publisher
Springer