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Clean production of microalgae high-value lipid fraction: Influence of different pretreatments on chemical and cytotoxic profiles of Chlorella vulgaris supercritical extracts and life cycle assessment

2025-02Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorVladic, Jelena
dc.contributor.authorRadman, Sanja
dc.contributor.authorJerkovic, Igor
dc.contributor.authorBesu, Irina
dc.contributor.authorSperanza, Lais Galileu
dc.contributor.authorHala, Ahmad Furqan
dc.contributor.authorKovacevic, Strahinja
dc.contributor.authorPerreira, Hugo
dc.contributor.authorGouveia, Luisa
dc.date.accessioned2025-03-21T15:28:21Z
dc.date.available2025-03-21T15:28:21Z
dc.date.issued2025-02
dc.description.abstractABSTRACT: Microalgae have emerged as a promising natural resource rich in bioactive compounds. Health-beneficial properties of microalgae, coupled with advantageous characteristics such as high biomass productivity, adaptability, robustness, and carbon dioxide mitigation, position them as a viable solution for global sustainable food production. This study explored clean and environmentally friendly processes to enhance the recovery of lipid bioactive fractions. Microwave (MW), enzymatic (ENZ), and ultrasound (US) pretreatments were applied to improve environmentally friendly extraction of lipid-based components using supercritical CO2. The effects of these pretreatments on extraction yield, chemical profiles, and cytotoxic properties of Chlorella vulgaris (Cv) and smooth C. vulgaris (sCv) extracts were investigated. Additionally, a Life Cycle Assessment (LCA) was conducted to evaluate environmental impacts. MW pretreatment achieved the highest yield increases, from 2.58 times (Cv) to 3.15 times (sCv). UHPLC-ESI-HRMS analysis revealed shifts in the distribution of pigments and derivatives caused by pretreatments, with ENZ extracts showing the most pronounced changes: pigments increased from 9.24% (control Cv) to 40.92% (Cv) and from 12.52% (control sCv) to 71.12% (sCv). Cv extracts exhibited greater activity against MDA-MB-453 cells, while sCv extracts from US pretreatment demonstrated the strongest effect on HeLa cells. The LCA indicated reduced environmental impacts of the pretreatment-enhanced processes up to 65% compared to the control. A scenario analysis was presented to show further possible impact reduction by recirculating the CO2 solvent and substituting the energy source. These findings provide valuable insights into sustainable and scalable green processes for recovering microalgal bioactive components.eng
dc.identifier.citationVladic, J., Radman, S., Zizak, Z., Besu, I., Jerkovic, I., Speranza, L.G., Hala, A.F., Kovacevic, S., Perreira, H. & Gouveia, L. (2025) Clean production of microalgae high-value lipid fraction: Influence of different pretreatments on chemical and cytotoxic profiles of Chlorella vulgaris supercritical extracts and life cycle assessment. In: Journal of Cleaner Prouction, 2025, vol. 491, article 144823. https://doi.org/10.1016/j.jclepro.2025.144823
dc.identifier.doi10.1016/j.jclepro.2025.144823
dc.identifier.issn0959-6526
dc.identifier.urihttp://hdl.handle.net/10400.9/5615
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.hasversionhttps://www.sciencedirect.com/journal/journal-of-cleaner-production
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectChlorella Vulgaris
dc.subjectSupercritical carbon dioxide
dc.subjectPretreatments
dc.subjectLife cycle assessment
dc.subjectGreen chemistry
dc.titleClean production of microalgae high-value lipid fraction: Influence of different pretreatments on chemical and cytotoxic profiles of Chlorella vulgaris supercritical extracts and life cycle assessmenteng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.citation.titleJournal of Cleaner Production
oaire.citation.volume491
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameVladic
person.familyNameJerkovic
person.familyNameGouveia
person.givenNameJelena
person.givenNameIgor
person.givenNameLuisa
person.identifierH-4046-2011
person.identifier.ciencia-id5E11-D9E4-6586
person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0002-3891-7266
person.identifier.orcid0000-0002-0727-6662
person.identifier.orcid0000-0003-0196-6360
person.identifier.ridD-6439-2017
person.identifier.scopus-author-id7004135834
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationb3da1cb4-4933-4d6e-9c11-69e69c370b05
relation.isAuthorOfPublicationa0543a90-955d-4d31-a1d0-5fec4e7e5366
relation.isAuthorOfPublication63dcbe08-14df-4886-98b7-2945cbe0e062
relation.isAuthorOfPublication.latestForDiscoveryb3da1cb4-4933-4d6e-9c11-69e69c370b05
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relation.isProjectOfPublication.latestForDiscovery29d85f88-1874-4fa0-b9d1-59c2c66e8c67

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