Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage

ABSTRACT: Fructophily is a rare trait that consists of the preference for fructose over other carbon sources. Here, we show that in a yeast lineage (the Wickerhamiella/Starmerella, W/S clade) comprised of fructophilic species thriving in the high-sugar floral niche, the acquisition of fructophily is concurrent with a wider remodeling of central carbon metabolism. Coupling comparative genomics with biochemical and genetic approaches, we gathered ample evidence for the loss of alcoholic fermentation in an ancestor of the W/S clade and subsequent reinstatement through either horizontal acquisition of homologous bacterial genes or modification of a pre-existing yeast gene. An enzyme required for sucrose assimilation was also acquired from bacteria, suggesting that the genetic novelties identified in the W/S clade may be related to adaptation to the high-sugar environment. This work shows how even central carbon metabolism can be remodeled by a surge of HGT events.

Keywords

Alcoholic fermentation Yeasts Fructophilic bacterium

URI

http://hdl.handle.net/10400.9/3051

Citation

Gonçalves, Carla; Wisecaver, Jennifer H.; Kominek, Jacek; [et.al.]. - Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage. In: eLife, 2018, Vol. 7, p. 1-28