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Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production

2024-03Journal article Open access
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dc.contributor.authorFontes, Afonso
dc.contributor.authorFrancisco, Ricardo
dc.contributor.authorFerreira, Frederico Castelo
dc.contributor.authorFaria, Nuno Torres
dc.contributor.authorMarques, Susana
dc.contributor.authorReis, Alberto
dc.contributor.authorMoura, Patrícia
dc.contributor.authorLukasik, Rafal M.
dc.contributor.authorSantos, José A. L.
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2024-04-17T11:19:49Z
dc.date.available2024-04-17T11:19:49Z
dc.date.issued2024-03
dc.description.abstractABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further cultivated in a bench bioreactor, using the same culture medium, under a batch regime. Flow cytometry was used to monitor the T. oleaginosus culture using the dual staining technique (SYBR Green and PI) for cell membrane integrity detection. Values of 42.28% (w/w) and 0.06 g/Lh lipid content and lipid productivity, respectively, were recorded for T. oleaginosus cultivated in the bench bioreactor operated under a batch regime. During the cultivation, most of the yeast cells maintained their integrity. T. oleaginosus has the potential to be used as an oil microbial source for a wide range of industrial applications. In addition, it is robust in adverse conditions such as lignocellulosic hydrolysate exposure and oxygen-limiting conditions. Flow cytometry is a powerful and useful tool for monitoring yeast cultivations on lignocellulosic hydrolysates for cell count, size, granularity, and membrane integrity detection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFontes, A., Francisco, R., Ferreira, F.C., Faria, N.T., Marques, S., Reis, A., Moura, P., Lukasik, R., Santos, J. & Silva, T.L. (2024) Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production. In: Processes, 2024, vol. 22 (3), article nº 558. https://doi.org/10.3390/pr12030558pt_PT
dc.identifier.doi10.3390/pr12030558pt_PT
dc.identifier.eissn2227-9717
dc.identifier.urihttp://hdl.handle.net/10400.9/4283
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationA FRONTrunner approacTransition to a circular & resilient future: deployment of systemic solutions with the support of local clusters and the development of regional community-based innovation schemes
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Bioengineering and Biosciences
dc.relation.publisherversionhttps://doi.org/10.3390/pr12030558pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOleaginous yeastpt_PT
dc.subjectLignocellulosic materialspt_PT
dc.subjectLipidspt_PT
dc.subjectFlow cytometrypt_PT
dc.titleMonitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA FRONTrunner approacTransition to a circular & resilient future: deployment of systemic solutions with the support of local clusters and the development of regional community-based innovation schemes
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/101037031/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT
oaire.citation.titleProcessespt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFerreira
person.familyNameTorres Faria
person.familyNameSantos Moita de Oliveira Marques
person.familyNameDelgado dos Reis
person.familyNameMoura
person.familyNameLukasik
person.familyNameSaraiva Lopes da Silva
person.givenNameFrederico Castelo
person.givenNameNuno Ricardo
person.givenNameSusana
person.givenNameAlberto José
person.givenNamePatrícia
person.givenNameRafal
person.givenNameMaria Teresa
person.identifier.ciencia-id4C18-FD61-0596
person.identifier.ciencia-id1D10-3461-3719
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idBA17-3D3E-5478
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0001-5177-6237
person.identifier.orcid0000-0002-4969-0028
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0001-9333-3231
person.identifier.orcid0000-0002-7805-5744
person.identifier.orcid0000-0003-4365-8868
person.identifier.ridK-8642-2012
person.identifier.ridA-8330-2015
person.identifier.ridA-6315-2011
person.identifier.scopus-author-id7103359012
person.identifier.scopus-author-id6506737268
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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