Repository logo
 
Publication

High cellulase-free xylanases production by Moesziomyces aphidis using low-cost carbon and nitrogen sources

2022-07Journal article Open access
Simple item page
dc.contributor.authorFaria, Nuno Torres
dc.contributor.authorMarques, Susana
dc.contributor.authorCerejo, Joana
dc.contributor.authorVorobieva, Ekaterina
dc.contributor.authorFonseca, César
dc.date.accessioned2022-07-27T15:30:44Z
dc.date.available2022-07-27T15:30:44Z
dc.date.issued2022-07
dc.description.abstractABSTRACT: Background Enzymes involved in xylan hydrolysis have several industrial applications. Selection of efficient microbial hosts and scalable bioreaction operations can lower enzyme production costs and contribute to their commercial deployment. This work aims at investigating the Moesziomyces aphidis yeast cultivation conditions that deliver maximal xylanase titres, yields and productivities using low-cost nitrogen (N) and carbon (C) sources. Results NaNO3 and KNO3 supplementation improved xylanase production 2.9- and 2.7-fold (against 67.2 U mL(-1)), respectively, using xylan as C source. Interestingly, the use of KNO3, instead of NaNO3, results in 2- to 3-fold higher specific activity, highlighting the potassium ion role. In addition, this study investigates synergetic effects on using ionic and organic N sources. A 4.9-fold increase in xylanase production, with high specific activity, is attained combining KNO3 and corn steep liquor (CSL). Exploring the previous findings, this study reports one of the highest extracellular xylanase production titres (864.7 U mL(-1)) by yeasts, using a media formulation containing dilute-acid pre-treated brewery spent grains (BSG), as C source and inducer, supplemented with KNO3 and CSL. Replacement of dilute-acid pre-treatmed BSG by untreated BSG had low impact on xylanase production, of only 6%. Conclusion Efficient production of M. aphidis xylanolytic enzymes, using low-cost N and C sources, is attractive for deployment of on-site enzyme production targeting different biotechnological applications under circular economy and biorefinery concepts. Potential xylanases end-users include industries such as brewing (using BSG as substrate for enzyme production), pulp and paper (benefiting from the cellulase-free xylanase activity) or lignocellulosic ethanol (for cellulase supplementation).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFaria, Nuno Torres... [et.al.] - High cellulase-free xylanases production by Moesziomyces aphidis using low-cost carbon and nitrogen sources. In: Journal of Chemical Technology and Biotechnology, 2022, Early Accesspt_PT
dc.identifier.doi10.1002/jctb.7172pt_PT
dc.identifier.eissn1097-4660
dc.identifier.issn0268-2575
dc.identifier.urihttp://hdl.handle.net/10400.9/3888
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationUIDB/BIO/04565/2020pt_PT
dc.relationUIDP/BIO/04565/2020pt_PT
dc.relationi4HB (LA/P/0104/2020)pt_PT
dc.relation.publisherversionhttps://doi.org/10.1002/jctb.7172pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectXylanasespt_PT
dc.subjectEnzyme productionpt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.subjectBiochemical engineeringpt_PT
dc.subjectCircular economypt_PT
dc.titleHigh cellulase-free xylanases production by Moesziomyces aphidis using low-cost carbon and nitrogen sourcespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-TEC%2F0696%2F2014/PT
oaire.citation.titleJournal of Chemical Technology and Biotechnologypt_PT
oaire.fundingStream3599-PPCDT
person.familyNameTorres Faria
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFonseca
person.givenNameNuno Ricardo
person.givenNameSusana
person.givenNameCésar
person.identifier284260
person.identifier.ciencia-id1D10-3461-3719
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.orcid0000-0002-4969-0028
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0003-2448-7063
person.identifier.ridA-6757-2010
person.identifier.scopus-author-id55955425000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication910ae041-d884-4346-b282-6ffe5ee43d6f
relation.isAuthorOfPublicationd700e60b-f720-42f6-be9a-b95bb080e262
relation.isAuthorOfPublication1c613a9f-bca3-465c-b261-8d67dfcaf6ce
relation.isAuthorOfPublication.latestForDiscoveryd700e60b-f720-42f6-be9a-b95bb080e262
relation.isProjectOfPublication904d7825-ae3e-4a9a-a976-595738789933
relation.isProjectOfPublication.latestForDiscovery904d7825-ae3e-4a9a-a976-595738789933

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
JournalChemTechnolBiotechnol_2022.pdf
Size:
493.23 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais