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Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes
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Orientador(es)
Resumo(s)
This work reports the development of a rapid flow cytometric method for the viability assessment of Gordonia alkanivorans strain 1B, a bacterium used in the biodesulfurization process. To demonstrate that it is possible to monitor by flow cytometric analysis changes in this bacterium physiological state, positive controls using the 5(6)-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining mixture were set. The loss of viability of G. alkanivorans resting cells in the presence of different concentrations of 2-hydroxybiphenyl, a very toxic end product of the dibenzotiophene desulfurization process, was assessed over a period of time. The results here reported demonstrate the potential of this technique for the biodesulfurization process monitoring and consequent enhancement.
Descrição
Palavras-chave
Biodesulfurization Fossil fuels Gordonia alkanivorans
Contexto Educativo
Citação
Teixeira, A.V.; Silva, T.P.; Silva, T. Lopes da; Paixão, S.M.; Alves, L. Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes. In: 1st International Congress on Bioenergy, Proceedings Book, Portalegre, Portugal, 23-25 Maio, 2013, 4p.