Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes

Teixeira, A. V.; Silva, Tiago; Silva, Teresa Lopes da; Paixão, Susana M.; Alves, Luís

http://hdl.handle.net/10400.9/2268

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
Proceeding Teixeira et al - 1st Int Cong on Bioenergy.pdf		317.06 KB	Adobe PDF	Download

Send Feedback

Authors

Teixeira, A. V.

Silva, Tiago

Silva, Teresa Lopes da

Paixão, Susana M.

Alves, Luís

Abstract(s)

This work reports the development of a rapid flow cytometric method for the viability assessment of Gordonia alkanivorans strain 1B, a bacterium used in the biodesulfurization process. To demonstrate that it is possible to monitor by flow cytometric analysis changes in this bacterium physiological state, positive controls using the 5(6)-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining mixture were set. The loss of viability of G. alkanivorans resting cells in the presence of different concentrations of 2-hydroxybiphenyl, a very toxic end product of the dibenzotiophene desulfurization process, was assessed over a period of time. The results here reported demonstrate the potential of this technique for the biodesulfurization process monitoring and consequent enhancement.

Keywords

Biodesulfurization Fossil fuels Gordonia alkanivorans

URI

http://hdl.handle.net/10400.9/2268

Citation

Teixeira, A.V.; Silva, T.P.; Silva, T. Lopes da; Paixão, S.M.; Alves, L. Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes. In: 1st International Congress on Bioenergy, Proceedings Book, Portalegre, Portugal, 23-25 Maio, 2013, 4p.