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Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes

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dc.contributor.authorTeixeira, A. V.
dc.contributor.authorSilva, Tiago
dc.contributor.authorSilva, Teresa Lopes da
dc.contributor.authorPaixão, Susana M.
dc.contributor.authorAlves, Luís
dc.date.accessioned2014-03-05T13:13:45Z
dc.date.available2014-03-05T13:13:45Z
dc.date.issued2013
dc.description.abstractThis work reports the development of a rapid flow cytometric method for the viability assessment of Gordonia alkanivorans strain 1B, a bacterium used in the biodesulfurization process. To demonstrate that it is possible to monitor by flow cytometric analysis changes in this bacterium physiological state, positive controls using the 5(6)-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining mixture were set. The loss of viability of G. alkanivorans resting cells in the presence of different concentrations of 2-hydroxybiphenyl, a very toxic end product of the dibenzotiophene desulfurization process, was assessed over a period of time. The results here reported demonstrate the potential of this technique for the biodesulfurization process monitoring and consequent enhancement.por
dc.identifier.citationTeixeira, A.V.; Silva, T.P.; Silva, T. Lopes da; Paixão, S.M.; Alves, L. Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes. In: 1st International Congress on Bioenergy, Proceedings Book, Portalegre, Portugal, 23-25 Maio, 2013, 4p.por
dc.identifier.urihttp://hdl.handle.net/10400.9/2268
dc.language.isoengpor
dc.subjectBiodesulfurizationpor
dc.subjectFossil fuelspor
dc.subjectGordonia alkanivoranspor
dc.titleFlow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processespor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortalegre, Portugalpor
oaire.citation.title1st International Congress on Bioenergypor
person.familyNameSilva
person.familyNameSaraiva Lopes da Silva
person.familyNamePaixão
person.familyNameAlves
person.givenNameTiago
person.givenNameMaria Teresa
person.givenNameSusana M.
person.givenNameLuís
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.ciencia-id561B-53A5-7359
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0003-4365-8868
person.identifier.orcid0000-0003-0955-4467
person.identifier.orcid0000-0001-6245-775X
person.identifier.scopus-author-id6603112228
person.identifier.scopus-author-id6701310833
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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relation.isAuthorOfPublication.latestForDiscovery4c11e382-9f04-42cc-9ca0-a4ade44298f1

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