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Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processing

2026-02Artigo científico Acesso aberto
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg11:Cidades e Comunidades Sustentáveis
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorMatias, João
dc.contributor.authorRodrigues, Thomas
dc.contributor.authorTorres, Cristiana A. V.
dc.contributor.authorMarques, Susana
dc.contributor.authorRibeiro, Belina
dc.contributor.authorGírio, Francisco
dc.contributor.authorReis, Maria A.
dc.contributor.authorFreitas, Filomena
dc.date.accessioned2026-03-10T15:32:32Z
dc.date.available2026-03-10T15:32:32Z
dc.date.issued2026-02
dc.description.abstractABSTRACT: This study integrates the valorization of a lignocellulose material into poly(3-hydroxybutyrate), P(3HB), with biopolymer extraction from bacterial cells with the enzyme alcalase. The work focused on Burkholderia thailandensis DSM 13276 as the P(3HB) producer and on eucalyptus bark, a byproduct from the pulp industry, as the sole feedstock for bacterial cultivation. The eucalyptus bark was hydrolyzed by a cellulolytic enzymatic cocktail following steam explosion and further subjected to ultrafiltration for enzyme recovery. The resulting hydrolysate supported good cell growth, achieving a cell dry weight of 7.67 +/- 0.16 g/L within 72 h of cultivation, and high P(3HB) content (60.0 +/- 2.19 wt %) in the bacterial cells, clearly favoring biopolymer synthesis over cell growth, as demonstrated by the polymer and growth yields (0.190 gP(3HB)/gsugar and 0.026 gX/gsugar, respectively). High extraction efficiency (96%) and biopolymer purity (100 +/- 3.38%) were reached by enzymatic treatment, resulting in a sample with properties aligned with those of commercial P(3HB) in terms of molecular mass distribution, crystallinity, and thermal properties. These findings demonstrate the successful use of a sustainable feedstock together with the application of environmentally friendly technologies based on the use of enzymes for both lignocellulosic saccharification and biopolymer recovery to develop high-quality bioplastics, advancing the goals of a circular bioeconomy.eng
dc.identifier.citationMatias, J., Rodrigues, T., Torres, C., Marques, S. Ribeiro, B., Gírio, F., Reis, M., & Freitas, F. (2026). Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processing. In: ACS Sustainable Chemistry & Engineering, 2026, vol. 14 (7), p. 3749-3757. https://doi.org/10.1021/acssuschemeng.5c14243
dc.identifier.doi10.1021/acssuschemeng.5c14243
dc.identifier.issn2168-0485
dc.identifier.urihttp://hdl.handle.net/10400.9/6297
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Chemical Society
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationDevelopment of PHA-based biocomposites with Bacterial Cellulose and CGC for textile and active packaging applications.
dc.relation.hasversionhttps://pubs.acs.org/doi/10.1021/acssuschemeng.5c14243?src=getftr&utm_source=clarivate&getft_integrator=clarivate
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEucalyptus bark hydrolysate
dc.subjectEucalyptus residues
dc.subjectEnzymatic hydrolysis
dc.subjectLignocellulosic residues
dc.subjectEnzymatic saccharification
dc.subjectWaste valorization
dc.subjectCircular bioeconomy
dc.titleSustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processingeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumber2023.03035.BD
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleDevelopment of PHA-based biocomposites with Bacterial Cellulose and CGC for textile and active packaging applications.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIhttp://hdl.handle.net/10400.9/5651
oaire.awardURIhttp://hdl.handle.net/10400.9/6296
oaire.citation.endPage3757
oaire.citation.issue7
oaire.citation.startPage3749
oaire.citation.titleACS Sustainable Chemistry & Engineering
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues
person.familyNameMarques
person.familyNameRibeiro
person.familyNameGírio
person.familyNameReis
person.familyNameFreitas
person.givenNameThomas
person.givenNameSusana
person.givenNameBelina
person.givenNameFrancisco
person.givenNameMaria A.
person.givenNameFilomena
person.identifier3508304
person.identifier.ciencia-id5B1E-CD89-C355
person.identifier.ciencia-id2813-8333-637F
person.identifier.ciencia-id9215-70D3-D42E
person.identifier.orcid0000-0002-3581-9505
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0001-5145-2486
person.identifier.orcid0000-0002-5376-8430
person.identifier.orcid0000-0003-4000-1836
person.identifier.orcid0000-0002-9430-4640
person.identifier.ridD-8303-2013
person.identifier.ridK-1828-2012
person.identifier.scopus-author-id7102676470
person.identifier.scopus-author-id36861977100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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