A carregar...
Publicação
Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processing
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.27 MB | Adobe PDF |
Orientador(es)
Resumo(s)
ABSTRACT: This study integrates the valorization of a lignocellulose material into poly(3-hydroxybutyrate), P(3HB), with biopolymer extraction from bacterial cells with the enzyme alcalase. The work focused on Burkholderia thailandensis DSM 13276 as the P(3HB) producer and on eucalyptus bark, a byproduct from the pulp industry, as the sole feedstock for bacterial cultivation. The eucalyptus bark was hydrolyzed by a cellulolytic enzymatic cocktail following steam explosion and further subjected to ultrafiltration for enzyme recovery. The resulting hydrolysate supported good cell growth, achieving a cell dry weight of 7.67 +/- 0.16 g/L within 72 h of cultivation, and high P(3HB) content (60.0 +/- 2.19 wt %) in the bacterial cells, clearly favoring biopolymer synthesis over cell growth, as demonstrated by the polymer and growth yields (0.190 gP(3HB)/gsugar and 0.026 gX/gsugar, respectively). High extraction efficiency (96%) and biopolymer purity (100 +/- 3.38%) were reached by enzymatic treatment, resulting in a sample with properties aligned with those of commercial P(3HB) in terms of molecular mass distribution, crystallinity, and thermal properties. These findings demonstrate the successful use of a sustainable feedstock together with the application of environmentally friendly technologies based on the use of enzymes for both lignocellulosic saccharification and biopolymer recovery to develop high-quality bioplastics, advancing the goals of a circular bioeconomy.
Descrição
Palavras-chave
Eucalyptus bark hydrolysate Eucalyptus residues Enzymatic hydrolysis Lignocellulosic residues Enzymatic saccharification Waste valorization Circular bioeconomy
Contexto Educativo
Citação
Matias, J., Rodrigues, T., Torres, C., Marques, S. Ribeiro, B., Gírio, F., Reis, M., & Freitas, F. (2026). Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processing. In: ACS Sustainable Chemistry & Engineering, 2026, vol. 14 (7), p. 3749-3757. https://doi.org/10.1021/acssuschemeng.5c14243
Editora
American Chemical Society