Repository logo
 
Publication

Saccharomyces cerevisiae and Dekkera bruxellensis interactions in alcoholic fermentations: growth and 4-ethylphenol production

2013Conference object Open access
Simple item page
dc.contributor.authorCoutinho, Rute
dc.contributor.authorBranco, Patricia
dc.contributor.authorMonteiro, Margarida
dc.contributor.authorMalfeito-Ferreira, Manuel
dc.contributor.authorAlbergaria, Helena
dc.date.accessioned2014-04-08T16:08:27Z
dc.date.available2014-04-08T16:08:27Z
dc.date.issued2013
dc.description.abstractThe yeast Dekkera/Brettanomyces bruxellensis can cause enormous economic losses both in wine industry and fuel-ethanol processes due to production of phenolic off-flavour compounds and low ethanol productivities. In winemaking this microbial hazard is usually tackled by the use of chemical preservatives such as sulphur dioxide. In spite of this, D. bruxellensis strains are frequently found in wines at low levels (ca 103 cells/ml) where they can metabolise residual sugars producing phenolic off-flavours compounds, such as 4-ethyl phenol. In the present work we investigated S. cerevisiae and D. bruxellensis interactions during alcoholic fermentations and evaluated the effectiveness of antimicrobial peptides secreted by S. cerevisiae to prevent growth of the main wine spoilage yeast and the production of 4-ethylphenol. Several fermentations were performed with single cultures of D. bruxellensis and mixed cultures of S. cerevisiae and D. bruxellensis, both in synthetic grape juice (SGJ) and grape must. Yeast growth (culturability and viability) and fermentation performance (i.e. sugars consumption, ethanol and 4-ethylphenol production) of those fermentations was accessed by different methods, namely by florescence in situ hybridization and flow cytometry. Results showed that S. cerevisiae significantly reduced the growth of D. bruxellensis and the production of 4-ethylphenol both in SGJ and grape must fermentations performed with mixed cultures. Moreover, our work also showed that antimicrobial peptides secreted by S. cerevisiae are effective to prevent growth of D. bruxellensis and production of phenolic off-flavor compounds in wine.por
dc.identifier.citationCoutinho, R.; Branco, P.; Monteiro, M.; Malfeito-Ferreira, M.; Albergaria, A. Saccharomyces cerevisiae and Dekkera bruxellensis interactions in alcoholic fermentations: growth and 4-ethylphenol production. In: MicroBiotec'13: Portuguese Congress of Microbiology and Biotechnology, Book of Abstracts, Aveiro, Portugal, 6-8 December, 2013, p. 94por
dc.identifier.urihttp://hdl.handle.net/10400.9/2397
dc.language.isoengpor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectDekkera bruxellensispor
dc.subjectAlcoholic fermentationpor
dc.titleSaccharomyces cerevisiae and Dekkera bruxellensis interactions in alcoholic fermentations: growth and 4-ethylphenol productionpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceAveiro, Portugalpor
oaire.citation.titleMicroBiotec'13: Portuguese Congress of Microbiology and Biotechnologypor
person.familyNameBatista Branco
person.familyNameMalfeito-Ferreira
person.familyNameAlbergaria
person.givenNamePatricia Alexandra
person.givenNameManuel
person.givenNameHelena
person.identifier1007473
person.identifier.ciencia-idF314-7279-6F0F
person.identifier.ciencia-id7313-2302-AAB5
person.identifier.ciencia-idFF10-6CD1-AF19
person.identifier.orcid0000-0002-1760-6899
person.identifier.orcid0000-0002-7985-963X
person.identifier.orcid0000-0002-9062-9827
person.identifier.ridB-3215-2019
person.identifier.ridA-8351-2015
person.identifier.scopus-author-id6602549195
person.identifier.scopus-author-id7801441357
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication5f614149-d826-4268-bdbf-bc3a938d5d42
relation.isAuthorOfPublicationcc1fda67-74e1-4659-b72e-0d815f7efaa3
relation.isAuthorOfPublication63df14e3-952f-4b43-b37c-3cc1238e0830
relation.isAuthorOfPublication.latestForDiscovery5f614149-d826-4268-bdbf-bc3a938d5d42

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Abstract_MicroBiotec13_RC.pdf
Size:
16.43 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Resumos em livros de actas