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Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes

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dc.contributor.authorTeixeira, A. V.
dc.contributor.authorSilva, Tiago
dc.contributor.authorSilva, Teresa Lopes da
dc.contributor.authorPaixão, Susana M.
dc.contributor.authorAlves, Luís
dc.date.accessioned2013-12-09T12:33:39Z
dc.date.available2013-12-09T12:33:39Z
dc.date.issued2013
dc.description.abstractThe most commonly method used for sulfur removal from fossil fuels is hydrodesulfurization, a physico-chemical process at very high temperatures and pressures. An alternative to this process is biodedesulfurization (BDS), a microbiological process that works at atmospheric pressure and temperature making it easier to work with and less expensive. It also as the advantage of easily desulfurizing recalcitrant sulfur compounds which are hard to remove by hydrodesulfurization [1]. Several bacteria species, such as Gordonia alkanivorans strain1B [2], are able to desulfurize dibenzothiophene, a model compound used commonly in BDS studies, to 2-hydroxybiphenyl (2-HBP) via the 4S pathway without destroying the carbon structure [3], therefore maintaining the fuel potential energy. BDS limitations are related with process parameters and with the cost of maintaining bacterial cultures so to enhance the BDS process, it is necessary to monitor how changes in the experimental system affect the microbial cells viability and consequently the process efficiency. An alternative method to conventional microbial techniques to determine cell viability is flow cytometry. This method provides a fast and accurate quantitative method for measurement of thousands of individual cells, based on scattered light and fluorescence emitted by specific dyes. The goal of this study was to develop a rapid method for viability assessment of G. alkanivorans cells using flow cytometry for further application to monitor and optimize BDS processes.por
dc.identifier.citationTeixeira, A.V.; Silva, T.P.; Silva, T. Lopes da; Paixão, S.M.; Alves, L. Flow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processes. In: 1st International Congress on Bioenergy, Book of Abstracts, Portalegre, Portugal, 23-25 Maio, 2013, p. 13por
dc.identifier.urihttp://hdl.handle.net/10400.9/2089
dc.language.isoengpor
dc.subjectFlow cytometrypor
dc.subjectGordonia alkanivoranspor
dc.subjectFossil fuelspor
dc.subjectBiodesulfurizationpor
dc.titleFlow cytometric method for cell viability evaluation of Gordonia alkanivorans strain 1B in fossil fuels biodesulfurization processespor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortalegre, Portugalpor
oaire.citation.title1st International Congress on Bioenergypor
person.familyNameSilva
person.familyNameSaraiva Lopes da Silva
person.familyNamePaixão
person.familyNameAlves
person.givenNameTiago
person.givenNameMaria Teresa
person.givenNameSusana M.
person.givenNameLuís
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.ciencia-id561B-53A5-7359
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0003-4365-8868
person.identifier.orcid0000-0003-0955-4467
person.identifier.orcid0000-0001-6245-775X
person.identifier.scopus-author-id6603112228
person.identifier.scopus-author-id6701310833
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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