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Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases

2023-02Journal article Open access
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dc.contributor.authorKommedal, Eirik G.
dc.contributor.authorAngeltveit, Camilla F.
dc.contributor.authorKlau, Leesa J.
dc.contributor.authorAyuso-Fernández, Iván
dc.contributor.authorArstad, Bjørnar
dc.contributor.authorAntonsen, Simen G.
dc.contributor.authorStenstrøm, Yngve
dc.contributor.authorEkeberg, Dag
dc.contributor.authorGírio, Francisco
dc.contributor.authorCarvalheiro, Florbela
dc.contributor.authorHorn, Svein J.
dc.contributor.authorAachmann, Finn Lillelund
dc.contributor.authorEijsink, Vincent G. H.
dc.date.accessioned2023-05-09T09:53:45Z
dc.date.available2023-05-09T09:53:45Z
dc.date.issued2023-02
dc.description.abstractABSTRACT: Degradation of plant biomass, comprised of cellulose and polyaromatic lignin, is promoted by light. Here, the authors show that light promotes lignin-catalyzed generation of hydrogen peroxide, which is used by redox enzymes to degrade cellulose. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight promotes microbial conversion of plant litter; this effect has been attributed to photochemical degradation of lignin, a major redox-active component of secondary plant cell walls that limits enzyme access to the cell wall carbohydrates. Here, we show that exposing lignin to visible light facilitates cellulose solubilization by promoting formation of H2O2 that fuels LPMO catalysis. Light-driven H2O2 formation is accompanied by oxidation of ring-conjugated olefins in the lignin, while LPMO-catalyzed oxidation of phenolic hydroxyls leads to the required priming reduction of the enzyme. The discovery that light-driven abiotic reactions in Nature can fuel H2O2-dependent redox enzymes involved in deconstructing lignocellulose may offer opportunities for bioprocessing and provides an enzymatic explanation for the known effect of visible light on biomass conversion.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationKommedal, Eirik G... [et.al.] - Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases. In: Nature Communications, 2023, vol. 14 (1), article nº 1063pt_PT
dc.identifier.doi10.1038/s41467-023-36660-4pt_PT
dc.identifier.eissn2041-1723
dc.identifier.urihttp://hdl.handle.net/10400.9/4047
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationUnravelling the secrets of Cu-based catalysts for C-H activation
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-023-36660-4pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLigninpt_PT
dc.subjectPolysaccharidespt_PT
dc.subjectLignocellulosic biomasspt_PT
dc.titleVisible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenasespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnravelling the secrets of Cu-based catalysts for C-H activation
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/856446/EU
oaire.citation.titleNature Communicationspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStreamH2020
person.familyNameFERREIRA GIRIO
person.familyNameCarvalheiro
person.givenNameFRANCISCO MANUEL
person.givenNameFlorbela
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.ciencia-id4016-9476-B97D
person.identifier.orcid0000-0002-5376-8430
person.identifier.orcid0000-0002-0270-4832
person.identifier.ridL-1860-2014
person.identifier.scopus-author-id6603247136
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication781a0b63-529e-4c34-8dcc-fd27e785e762
relation.isAuthorOfPublicationcdbcb5eb-94de-49eb-920b-089980c34cbf
relation.isAuthorOfPublication.latestForDiscoverycdbcb5eb-94de-49eb-920b-089980c34cbf
relation.isProjectOfPublication6d5e02da-f17e-47df-afc3-f29c289c5a6c
relation.isProjectOfPublication.latestForDiscovery6d5e02da-f17e-47df-afc3-f29c289c5a6c

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