Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii

Moniz, Patricia; Silva, Carla; Oliveira, Ana Cristina; Reis, Alberto; Silva, Teresa Lopes da

http://hdl.handle.net/10400.9/3623

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Authors

Moniz, Patricia

Silva, Carla

Oliveira, Ana Cristina

Reis, Alberto

Silva, Teresa Lopes da

Abstract(s)

ABSTRACT: Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) and 0.016 g L−1 h−1, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.

Keywords

Alternative carbon source Microalgae Lipids Flow cytometry

URI

http://hdl.handle.net/10400.9/3623

Citation

Moniz, Patrícia... [et.al.] - Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii. In: Processes, 2021, Vol. 9 (11), article nº 2005