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The role of heterotrophic microalgae in waste conversion to biofuels and bioproducts
Publication . Silva, Teresa Lopes da; Moniz, Patricia; Silva, Carla; Reis, Alberto
ABSTRACT: In the last few decades, microalgae have attracted attention from the scientific community worldwide, being considered a promising feedstock for renewable energy production, as well as for a wide range of high value-added products such as pigments and poly-unsaturated fatty acids for pharmaceutical, nutraceutical, food, and cosmetic markets. Despite the investments in microalgae biotechnology to date, the major obstacle to its wide commercialization is the high cost of microalgal biomass production and expensive product extraction steps. One way to reduce the microalgae production costs is the use of low-cost feedstock for microalgae production. Some wastes contain organic and inorganic components that may serve as nutrients for algal growth, decreasing the culture media cost and, thus, the overall process costs. Most of the research studies on microalgae waste treatment use autotrophic and mixotrophic microalgae growth. Research on heterotrophic microalgae to treat wastes is still scarce, although this cultivation mode shows several benefits over the others, such as higher organic carbon load tolerance, intracellular products production, and stability in production all year round, regardless of the location and climate. In this review article, the use of heterotrophic microalgae to simultaneously treat wastes and produce high value-added bioproducts and biofuels will be discussed, critically analyzing the most recent research done in this area so far and envisioning the use of this approach to a commercial scale in the near future.
Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
Publication . Taborda, Tiago; Moniz, Patricia; Reis, Alberto; Silva, Teresa Lopes da
ABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii
Publication . Moniz, Patricia; Silva, Carla; Oliveira, Ana Cristina; Reis, Alberto; Silva, Teresa Lopes da
ABSTRACT: Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) and 0.016 g L−1 h−1, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.
Cascading Crypthecodinium cohnii biorefinery: global warming potential and techno-economic assessment
Publication . Silva, Carla; Moniz, Patricia; Oliveira, Ana Cristina; Vercelli, Samuela; Reis, Alberto; Silva, Teresa Lopes da
ABSTRACT: Prior to the commissioning of a new industrial biorefinery it is deemed necessary to evaluate if the new project will be beneficial or detrimental to climate change, one of the main drivers for the sustainable development goals (SDG) of the United Nations. In particular, how SDG 7, Clean and Efficient Energy, SDG 3, Good Health and Well Being, SDG 9, Industry Innovation and Infrastructure, and SDG 12, Responsible Production and Consumption, would engage in a new biorefinery design, beneficial to climate change, i.e., fostering SDG 13, Climate Action. This study uses life cycle assessment methodology (LCA) to delve in detail into the Global Warming Impact category, project scenario GHG savings, using a conventional and a dynamic emission flux approach until 2060 (30-year lifetime). Water, heat and electricity circularity are in place by using a water recirculation process and a combined heat and power unit (CHP). A new historical approach to derive low and higher-end commodity prices (chemicals, electricity, heat, jet/maritime fuel, DHA, N-fertilizer) is used for the calculation of the economic indicators: Return of investment (ROI) and inflation-adjusted return (IAR), based upon the consumer price index (CPI). Main conclusions are: supercritical fluid extraction is the hotspot of energy consumption; C. cohnii bio-oil without DHA has higher sulfur concentration than crude oil based jet fuel requiring desulfurization, however the sulfur levels are compatible with maritime fuels; starting its operation in 2030, by 2100 an overall GHG savings of 73% (conventional LCA approach) or 85% (dynamic LCA approach) is projected; economic feasibility for oil productivity and content of 0.14 g/L/h and 27% (w/w) oil content, respectively (of which 31% is DHA), occurs for DHA-cost 100 times higher than reference fish oil based DHA; however future genetic engineering achieving 0.4 g/L/h and 70% (w/w) oil content (of which 31% is DHA), reduces the threshold to 20 times higher cost than reference fish oil based DHA; N-fertilizer, district heating and jet fuel may have similar values then their fossil counterparts.
Fermentation Wastes from Chrypthecodinium cohnii Lipid Production for Energy Recovery by Anaerobic Digestion
Publication . Eusebio, Ana; Moniz, Patricia; Silva, Teresa Lopes da; Marques, Isabel Paula
ABSTRACT: Wastes generated during the cultivation of marine microalga Crypthecodinium cohnii and after the lipid extraction process, were energetically valorized into biogas production through anaerobic digestion (AD). The tested wastes were extracted microalgae (Ae) with hexane (AeH) using supercritical extraction methods (AeS) and the supernatant obtained after culture medium centrifugation (M). The digestion of the algae biomass in the admixture with the supernatant medium (AeH+M+I and AeS+M+I) provided a higher methane content and a higher methane yield (582 and 440 L CH4/kg VS) than the substrates Ae and M, individually digested (155 and 96 L CH4/kg VS, respectively). Flow cytometry monitoring processes during AD indicated that the yield of the accumulated biogas was influenced by the operating conditions. The mixture of AeH+M+I was the only assay with a proportion of cells with less damaged membranes after AD, providing the highest methane yield and productivity (582 L CH4/kg VS and 31 L CH4/kg VS.d, respectively) and the highest energetic potential of 5.8 KWh/kg VS of all the substrates. From the results, AD integration to lipid production by C. cohnii to recover energy from the generated wastes enhanced the sustainability of the entire process and promoted the practice of zero waste.
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
3599-PPCDT
Funding Award Number
PTDC/EAM-AMB/30169/2017