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Research Project
A FRONTrunner approacTransition to a circular & resilient future: deployment of systemic solutions with the support of local clusters and the development of regional community-based innovation schemes
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Publications
Mapping municipal solid waste to boost circular valorization practices in Łódzkie
Publication . Moura, Patrícia; Eusebio, Ana; Patinha, Pedro; Quental, Lídia; Lukasik, Rafal M.
ABSTRACT: Geographic Information System (GIS) is a powerful instrument that can be used for the spatial representation of waste and by-product flows at various levels, allowing to improve municipal solid waste (MSW) management. The mapping obtained can be advantageously targeted to build a regional network of technological, economic, social and environmental linkages and to boost circular economy practices. In this work, the data on MSW produced in the Łódzkie region, Poland, during 2021 were used to generate a geolocalized database and an interactive web map, using ArcGIS software. The geodatabase and the map visualization were organized in three layers of information with increasing detail to foster a map-driven symbiosis between waste suppliers and waste recipients, paving the way for a more circular regional economy.
Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts
Publication . Silva, Teresa Lopes da; Fontes, Afonso; Reis, Alberto; Silva, Carla; Gírio, Francisco
ABSTRACT: The world climate crisis has triggered the search for renewable energy sources. Oleaginous yeasts are a potential renewable source of biofuels. However, the yeast-derived biofuels cost is still non-competitive with the fossil fuel prices. To improve the sustainability of yeast-derived biofuels, it is necessary to valorize all yeast biomass fractions, an approach based on the biorefinery concept. This review describes the present situation of the oleaginous yeast biorefinery research, emphasizing the feedstock, processes and techniques involved in this concept, as well as on potential bioproducts that can be obtained from oleaginous yeast biomass.
Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production
Publication . Fontes, Afonso; Francisco, Ricardo; Ferreira, Frederico Castelo; Faria, Nuno Torres; Marques, Susana; Reis, Alberto; Moura, Patrícia; Lukasik, Rafal M.; Santos, José A. L.; Silva, Teresa Lopes da
ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further cultivated in a bench bioreactor, using the same culture medium, under a batch regime. Flow cytometry was used to monitor the T. oleaginosus culture using the dual staining technique (SYBR Green and PI) for cell membrane integrity detection. Values of 42.28% (w/w) and 0.06 g/Lh lipid content and lipid productivity, respectively, were recorded for T. oleaginosus cultivated in the bench bioreactor operated under a batch regime. During the cultivation, most of the yeast cells maintained their integrity. T. oleaginosus has the potential to be used as an oil microbial source for a wide range of industrial applications. In addition, it is robust in adverse conditions such as lignocellulosic hydrolysate exposure and oxygen-limiting conditions. Flow cytometry is a powerful and useful tool for monitoring yeast cultivations on lignocellulosic hydrolysates for cell count, size, granularity, and membrane integrity detection.
Development of the operational model and methodology of collecting data, updating, and sharing methodologies to specific groups of stakeholders
Publication . Kochanska, Ewa; Adamkiewicz, Iwona; Wozniak, Katarzyna; Przygodzki, Zbigniew; Trippner-Hrabi, Justyna; Feltynowski, Marcin; Szafranska, Ewa; Jeziorska-Biel, Pamela; Nalej, Marta; Jażdżewska, Iwona; Adamus, Jagoda; Chadzyński, Jacek; Podgórski, Marcin; Bielinski, Dariusz; Siciński, Mariusz; Gozdek, Tomasz; Wręczycki, Jakub; Okraska, Michal; Benedetti, Vittoria; Menin, Lorenzo; Patuzzi, Francesco; Baratieri, Marco; Peppas, Antonis; Politi, Chrysa; Skendera, Doris; Lenssen, Jip; Turati, Daniele; Ravaglia, Pieter; Imiela, Mateusz; Eusebio, Ana; Reis, Alberto; Sousa Rocha, Cristina; Camocho, David; Alexandre, Jorge; Quental, Lídia; Moura, Patrícia; Patinha, Pedro; Lukasik, Rafal M.; Wozniak, Patrycja; Tyczkowski, Adam; Bergamin, Sara; Waszak, Lukasz; Laskowska, Agnieszka; Mirys, Magdalena; Pakowska, Anna; Kałucka, Diana; Straszyńska-Pięta, Katarzyna; Rocha, Monike; Pascale, Carmine; Meletis, Konstantinos; Kehri, Roula; Gazis, Nikos; Stamatakis, Andreas; Bos, Sander; Fonseca, Graca; Cabral, Alexandra; Wojdal, Laila
ABSTRACT: The overall objective of the FRONTSH1P project is to ensure the green and just transition of the Polish Lodzkie Region towards decarbonization and territorial regeneration through demonstration at TRL7 of four Circular Systemic Solutions (CSS), interconnected one each other and facing the identified regional challenges/opportunities. FRONTSH1P systemic approach will be enabled by a circular governance model that connects environmental policies with social justice through just transition ensuring environmental sustainability, jobs and social inclusion. The flexibility and modularity of the four CSSs guarantee a high replicability and scalability to other territories across Europe and beyond and this will be demonstrated with the involvement of four additional Regions across EU. This report – Deliverable 2.2 (D2.2) is the result of the work carried out under the Work Package 2 - Regional Systemic Circular Economic Approach, Task 2.2. - Regional Circularity Booster Toolkit. D2.2 meets the FRONTSH1P key enabler and requirement, presents the initial approach of the digital platform creation and methodology for data collection and sharing scheme elaboration. The EU General Data Protection Regulation and open data compliant frameworks have been addressed in D2.2 too.
Optimizing deep eutectic solvent pretreatment for enhanced glucan recovery from miscanthus
Publication . Piedade, Patrícia J.; Nowotarski, Michal M.; Dudek, Gabriela; Lukasik, Rafal M.
ABSTRACT: This work focuses on the use of the deep eutectic solvent composed of choline chloride and acetic acid (1 : 2) in the pretreatment of the perennial energy crop Miscanthus, commonly called silvergrass. The pretreatment time and temperature were optimized to achieve a maximum of the glucan and minimum of the lignin contents in the pretreated biomass. In the optimization approach, the maximal glucan recovery in the pretreated solid was also considered. The performed optimization resulted in conditions (2 h 52 min and 150 degrees C), at which a pretreated biomass contained 74.1 wt% and 9.5 wt% of glucan and lignin respectively, and a glucan recovery was as high as 87.0 wt%. Furthermore, the biocompatibility of deep eutectic solvent was evaluated by using enzymatic hydrolysis washed and unwashed pretreated biomass produced at optimal conditions. The enzymatic hydrolysis of washed biomass resulted in higher glucan and xylan conversion than those achieved from unwashed biomass, deeming the step of biomass washing necessary. This was confirmed by the fractal kinetics modelling that confirmed higher accessibility of glucan for washed biomass than for unwashed Miscanthus sample.
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Funding agency
European Commission
Funding programme
H2020
Funding Award Number
101037031